Detection and typing of molluscum contagiosum virus in skin lesions by using a simple lysis method and polymerase chain reaction

Post-kala-azar dermal leishmaniasis (PKDL) is very uncommon among HIV-positive patients, and very few cases have so far been documented. A case of atypical disseminated leishmaniasis resembling PKDL in an HIV-positive patient successfully treated with N-methylglucamine antimoniate is reported. The polymerase chain reaction performed on the skin lesions was positive for Leishmania infantum.

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Detection of herpesvirus DNA in peripheral blood mononuclear cells and skin lesions of patients with pemphigus by polymerase chain reaction

British Journal of Dermatology ◽

10.1046/j.1365-2133.1999.03201.x ◽

1999 ◽

Vol 141 (6) ◽

pp. 1033-1039 ◽

Cited By ~ 39

Author(s):

M.A. Tufano ◽

A. Baroni ◽

E. Buommino ◽

E. Ruocco ◽

M.L. Lombardi ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Skin Lesions ◽

Chain Reaction ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Polymerase Chain

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Sweet’s syndrome associated with clonal hematopoiesis of indeterminate potential responsive to 5-azacitidine

Therapeutic Advances in Hematology ◽

10.1177/2040620716680330 ◽

2016 ◽

Vol 8 (2) ◽

pp. 91-95 ◽

Cited By ~ 2

Author(s):

George Yaghmour ◽

Eric Wiedower ◽

Bassam Yaghmour ◽

Sara Nunnery ◽

Eric Duncavage ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Rare Condition ◽

Skin Lesions ◽

Sweet’S Syndrome ◽

Sequence Variant ◽

Chain Reaction ◽

Sweet's Syndrome ◽

Clonal Hematopoiesis ◽

Myeloid Neoplasms ◽

Polymerase Chain

Sweet’s syndrome (SS) is a rare condition characterized by the abrupt appearance of painful skin lesions due to neutrophilic dermal infiltration. Hematologic neoplasms, particularly acute myeloid leukemia (AML) and myelodysplastic syndromes (MDSs), have been commonly reported in association with SS. Clonal hematopoiesis of indeterminate potential (CHIP) is an emerging entity that is a precursor state to myeloid neoplasms. CHIP has not been previously associated with SS. We report the case of a 71-year-old man who presented with recurrent, painful edematous and erythematous papules and nodules for 18 months despite treatment with corticosteroids. He had normal blood counts, but a macrocytosis was noted (110 fl). Alternative causes of macrocytosis were ruled out. A skin biopsy confirmed a diagnosis of SS. Bone marrow biopsy specimen yielded a normal karyotype except for loss of the Y chromosome and equivocal morphologic findings. Polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR) of selected genes from the peripheral blood demonstrated a mixed lineage leukemia (MLL) partial tandem duplication (PTD) and sequence variant in CCAAT/enhancer binding protein alpha (CEBPA). These findings were consistent with a diagnosis of CHIP. The patient was treated with 5-azacitidine and achieved a complete remission of his skin lesions and was able to discontinue corticosteroids. To our knowledge, this is the first report of a patient with recurrent SS associated with CHIP. In addition to other myeloid neoplasms like AML and MDS, CHIP should be considered as a potential etiology in cases of recurrent SS. Treatment with a hypomethylating agents such as azacitidine could also serve as an alternative to systemic corticosteroid therapy.

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Association between erythema nodosum/ erythema induratum of Bazin and Mycobacterium tuberculosis infection in Koreans

Indian Journal of Dermatology Venereology and Leprology ◽

10.25259/ijdvl_958_18 ◽

2021 ◽

Vol 0 ◽

pp. 1-5

Author(s):

Kang Su Kim ◽

Jae Seok Kim ◽

Sang Seok Kim ◽

Chul Woo Kim

Keyword(s):

Polymerase Chain Reaction ◽

Mycobacterium Tuberculosis ◽

Erythema Nodosum ◽

Lower Extremities ◽

Skin Lesions ◽

Reaction Products ◽

Chain Reaction ◽

Mycobacterium Tuberculosis Infection ◽

Nested Polymerase Chain Reaction ◽

Polymerase Chain

Background: Erythema nodosum and erythema induratum of Bazin are similar inflammatory diseases of the lower extremities. These are clinically distinguishable entities, though overlap can occur. Both diseases are reported to be related to Mycobacterium tuberculosis infection, but it is very difficult to identify Mycobacterium tuberculosis in skin lesions. Aim: This study aimed to develop a new nested polymerase chain reaction targeting the IS6110 insertion sequence of M. tuberculosis to improve the M. tuberculosis detection rate in skin lesions of erythema nodosum or erythema induratum of Bazin. Methods: From May 2016 to Jan 2018, 14 patients with clinically suspicious erythema nodosum or erythema induratum were enrolled in the study. Two cases were classified as erythema nodosum and 12 as erythema induratum. Individual patients were subjected to a 4-mm punch biopsy, and their venous whole blood was sampled immediately after diagnosis. Results: Eight patients were tested for M. tuberculosis using QuantiFERON, of which seven (87.5%) were positive. IS6110-nested polymerase chain reaction on all 14 patients identified 11 (78.6%) positive cases. Four of the eight (50%) individuals tested with QuantiFERON were also positive in the IS6110 nested polymerase chain reaction. The difference between the outcomes of the QuantiFERON and the IS6110-nested polymerase chain reaction tests was not statistically significant. There was also no significant agreement between the results of both assays. Sequencing the IS6110-nested polymerase chain reaction products showed a 97%–100% nucleotide sequence identity with the H37Rv genome. Conclusion: It is important to test for tuberculosis in patients with multiple tender subcutaneous nodules on their lower extremities in high-burden tuberculosis countries like Korea. Limitations: We need to register more suspicious patients to verify the association between erythema nodosum/erythema induratum of Bazin and M. tuberculosis. Furthermore, it is necessary to improve the more sensitive polymerase chain reaction technique to identify M. tuberculosis directly in cutaneous lesions.

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